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KMID : 1123920020160020343
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Korean Journal of Oriental Physiology and Pathology
2002 Volume.16 No. 2 p.343 ~ p.347
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Effects of Gamibojungikki-tang on LDH activity of Cultured Spinal Sensory Neurons
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Lee Chang-Ho
Kwon Kang-Beom
Park Jun-Su
Song Yung-Sun
Ryu Do-Gon
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Abstract
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In order to darify the neuroprotective effect of Gamibojungikki-tang(GBJIKT) water extract on cultured mouse spinal sensory neuron damaged by glucose Oxidase (GO), NR (Neutral Red) assay and LDH (Lactate Dehydrogenase) activity assay were carried out after the cultured mouse spinal sensory neuron were preincubated with various concentrations of GBJIKT water extract for 3 hours prior to exposure of GO. Cell viability of cultured mouse spinal sensory neurons exposed to various concentrations of GO for 8 hours was decreased in a dose-dependent manner. NR/sub 50/ values were 50 mU/ml GO. Cultured mouse spinal sensory neurons in the medium containing various concentration of GO for 8 hours showed increasing of LDH activity. We knew that GO was toxic on cultured spinal sensory neurons. Pretreatment of GBJIKT water extract for 3 hours following GO prevented the GO-induced neurotoxicity such as increasing of LDH activity. These results suggest that GO shows toxic effect on cultured spinal sensory neurons and GBJIKT water extract is highly effective in proecting the neurotoxicity induced by GO.
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KEYWORD
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Gamibojungikki-tang(加味補中益氣湯), Mouse Spinal Sensory Neuron, Glucose Oxidase, Neurotoxicity, Lactate dehydrogenase activity
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